https://dreruplab.com/home daily 1.0 2021-12-20 https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1592790796270-0JBWRHY66VHJEL5WYLPM/zebrafish+whole+larva.jpg Home We use zebrafish posterior lateral line (pLL) neurons to study intracellular transport in neurons. The cell bodies of these neurons lie in the pLL ganglion just behind the ear (left). Their axons extend through the trunk and branch into axon terminals that innervate sensory organs (right; sensory cells not shown). Using transient transgenesis, we can mark cargo such as mitochondria in single neurons in live larvae to analyze their movement (shown above in magenta; arrowheads point to individual organelles in insets). Middle inset shows individual mitochondria that move over several seconds (arrows). https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593797740829-W5CNPWBBXDA87OPZJENG/drerup+lab+logo+final.png Home Lab logo by Serena Wisner 2020 https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1592775519234-3AKKQCOO8IMI82ADQ4LV/2dpf+mito+wt.gif Home Mitochondrial transport in a zebrafish axon. Using mosaic expression of mRFP localized to mitochondria, movement of individual organelles can be recorded and then analyzed to study transport dynamics. Scale bar is 10 um. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593796263507-H3NYCVVJKQ4VBKV6AY1N/drerup%2Blab%2Blogo%2Bfinal.jpg Home https://dreruplab.com/people daily 0.75 2025-10-29 https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/efc3db93-200b-4df3-9342-07143bc9607a/IMG_6850.JPG People - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/b9c31580-712b-4b6b-912b-75fa3ff4e654/IMG_6356.jpg People - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/8b8a21e0-05fb-4042-ba19-342f373d9a0a/Drerup+image.jpg People https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/d46308d2-007c-41fe-abdd-b9691c600a77/DSC_0962.jpg People - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/97047310-4f3e-42b0-b90f-45c8153d1631/Chris+Stein-4097.jpg People - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/56bb39e1-eade-4060-b399-8707e4ab114f/cc47fd1d-bf66-40a1-bd35-71fcd9898e0d%7E1.jpg People - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/44eb628b-f5c0-483f-aeb4-08be02afd999/DSC07952.jpg People - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://dreruplab.com/projects daily 0.75 2020-07-01 https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593120356541-L2XBYHQ9C2T1T7W1JQOO/nudc+microtubule+network.jpg Projects In wildtype larvae, axon terminals are finely branched, creating a basket like structure (top left). Acetylated tubulin staining in wildtype (top right) shows linear arrays of stabilized microtubules that enter the terminal. In our novel mutant line (bottom), axon terminals are swollen and contain an expanded network of stabilized microtubules (arrow), a phenotype associated with enhanced dynein activity. (Images by Kate Pinter and Dane Kawano). https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593092447511-DBCTU277F56KN50YPC55/eb3+movie.gif Projects Imaging microtubule dynamics in vivo in a zebrafish axon. EB3-GFP marks the plus (fast growing) end of microtubules. As microtubules grow, the “comets” can be used to assay distance and velocity of microtubule growth. (Video by Dane Kawano) Credit to D. Kawano https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593217648312-TJXDY1MGI0GQYON65O2K/3D+mito+hb9.jpg Projects This is a mid-section of the trunk of a zebrafish larva at 5 dpf. The arrowhead points to an axon terminal of the lateral line sensory axons. Arrows point to motor neuron axons that exit the spinal cord (top). https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593218737214-5DTPWHARO4EGFL0BXIGK/timelapse+merge+larger.gif Projects Photoconversion was used to label mitochondria in axon terminals and then these converted (magenta) organelles were tracked over time. This analysis revealed that 50 % of old (magenta) organelles had vacated the axon terminal within a few hours and new (green) organelles had replaced them. (Video by Amrita Mandal) https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593094656640-83Q9J3J8K2UU6ZZBCXJ1/Mito+load+figure.jpg Projects Mitochondria can be labeled in individual neurons of the lateral line ganglia (magenta above, white below; arrows). This allows for analysis of mitochondrial load in the cell bodies and corresponding axon terminals. When retrograde transport is disrupted in this mutant line, axon terminals accumulate mitochondria at the expense of the cell body. Mitochondrial load - mitochondrial area/area of the neuron analyzed. Scale bars - 10um. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593218069937-Y830IG60AH2GTS56RT03/lln+mutants.jpg Projects Four of the novel mutant lines identified to date. Compared to wildtype (top), all mutants develop normally through larval stages (5 days post-fertilization (dpf) shown). However, axon terminals are swollen (arrowheads; right), a sign of disrupted retrograde transport. To visualize the neurons, GFP is expressed in the neuronal cytoplasm and mitochondria are labeled with mRFP in motor neuron axons. https://dreruplab.com/publications daily 0.75 2025-10-29 https://dreruplab.com/lab-news-and-lab-fun daily 0.75 2025-10-29 https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1629985875375-AMSKXHLLFEFYQXXH5MFB/IMG_3571.JPG Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/38cce6a3-fc47-42a7-a227-1b804ae44da9/IMG_4876.JPG Lab News and Lab Fun! - Make it stand out Don’s poster illustrated the disruption to the endolysosmal pathway in vps18 mutant zebrafish neurons https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/cdcce172-8025-4f0e-acc7-4c1588e538fc/IMG_4436.JPG Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/bd5375ff-2f48-4211-b905-cbe61b4c1db6/Image+from+iOS.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1592781674133-WLEFOM1ZCPQJR3GNE9C4/IMG_1946.jpg Lab News and Lab Fun! Katie, Amrita, and Serena at the American Society for Cell Biology meeting in Washington DC. Fall 2019. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1592783869601-8UYY2R6PTODKV651PFCV/IMG_1669.jpg Lab News and Lab Fun! SDB 2019 in Boston! Dane, Katie, Serena, and Amrita enjoyed some classic Irish fare before a stellar poster session. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/8df4f523-38e4-4e4f-8f5c-b22db1a5db05/Image+from+iOS+%281%29.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/4f474e82-16b8-427c-b50d-eaf267bb3bda/IMG_6197.jpg Lab News and Lab Fun! - Make it stand out Left to Right: Brittany Salazar, Serena Wisner, Tia Endrizzi, Candy Wong, Jacob Edwards, Chris Stein, Rachel Jones, Angelica Lang https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/b0e6f722-0516-4820-aa54-c2920f866951/IMG_5528.JPG Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/b76457b4-322c-4b33-9e3d-bb9e960681c6/IMG_4853.JPG Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/95d793cb-82e9-4b21-8714-32e186ac617b/IMG_4423.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/d68edf9a-e7ee-48dd-bc4e-0b1c4ac4e8ca/IMG_7174.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/d232d7aa-0aee-4dcb-8aa4-51dc016942ee/20231203_192540.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/8503505b-92b8-4986-8abb-5d7230023270/halloween+party.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/4a629482-8a36-4c6d-810b-411724db06c8/IMG_6006.PNG Lab News and Lab Fun! - Make it stand out Left to Right: Brooke Weiler, Angelica Lang, Serena Wisner, Chris Stein https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/90586c75-6290-49dd-aaa3-c5d5ed63dc9e/lab+and+the+lake+1.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/68784699-fffd-4c13-84f2-f34344b48db9/20230630_155437.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/6b82730d-2029-41db-a3c3-f8184b095b86/IMG_7375.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/3ff6a140-0a67-4872-8510-07c5d3e714e1/IMG_7149.jpeg Lab News and Lab Fun! - Make it stand out Angelica presents her work at IZFS https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/467a43af-9c66-4b04-8068-2aee765edf91/holiday+party+fun.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1592781912310-H928P9CUOU465M8YOYJA/IMG_1500.jpg Lab News and Lab Fun! Goodbye party for Wesley before her trip to Arizona for graduate school, summer 2019. Left to right: Rebecca, Kate, Dane, Katie, Serena, Amrita, and Wesley. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/7fc7b276-3d83-496b-820c-d7581d005fb0/IMG_5208.JPG Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/fc689ce5-eee3-4198-a289-bb2e5f62d721/IMG_4889.JPG Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/4fcbe4dc-0c0c-4c48-bb46-e13a40f34ca5/lab+at+the+lake+2.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/e92bbd6b-cecc-4b0a-9cd3-cbd477cb2ea5/IMG_7155.jpeg Lab News and Lab Fun! - Make it stand out Roger presents his work at IZFS https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/db1ac29f-5df1-4669-91c2-54c5987f2061/lab+picture+at+the+lake.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/2a0ccd8e-ecae-437b-8291-d4adaf2c8040/IMG_5525.JPG Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/08a5ebcc-27c4-4e5d-b0d9-4b0df0993512/sw+holiday+party.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593288866785-3FV5T3UR23WGTE3EI7FA/2880px-Madison_Picnic_Point.jpg Lab News and Lab Fun! https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1592790716283-NVTTJKMEYOIX3EPLAX48/IMG_1965.jpg Lab News and Lab Fun! Lab Holiday Party December of 2019. From left to right: Katie Klier, Serena Wisner, Amrita Mandal, Katie Drerup, Kate Pinter. Front: Lea the lab lab. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1595678046802-L1P2D5KYPZEKCPLDSYDI/Screen+Shot+2020-07-24+at+7.29.34+PM.png Lab News and Lab Fun! Lab Happy Hour/Goodbye party as folks move on to new labs, new schools, and new adventures (July 24,2020). So lucky this awesome group chose to work with me. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/788eb6c8-5fe1-46d2-a357-55d91a7b11ea/IMG_7152.jpeg Lab News and Lab Fun! - Make it stand out Serena presents her work at IZFS https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/4d858f84-a99c-445c-b8d7-fd4233076d92/IMG_5548.JPG Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/30b65f63-fff0-4bd2-9431-0862a261e6e6/Chris+Stein-4097.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/e7d418ab-9b6b-4fe0-b55b-de37576e24fb/IMG_4885.JPG Lab News and Lab Fun! - Make it stand out Mady showing off her work on the HOPs complex in neurons https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593268981007-259IQETJSSJ4OTBBX613/IMG_0741.jpg Lab News and Lab Fun! https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/8c02ad9e-53f3-4ffb-b1a3-612023198215/IMG_7539.jpg Lab News and Lab Fun! - Make it stand out L to R: Muhammad, Roger, Brooke, Katie, Chris, Serena (top), Brittany, and Angelica https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/7e13c48f-1563-46c1-82a9-140a8040a875/IMG_7344.jpg Lab News and Lab Fun! - Make it stand out L to R: Chris Stein, Brooke Weiler, Serena Wisner, Brittany Salazar, Angelica Lang, Roger Schultz https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/61f9ed22-5929-45e9-8fa1-69ec90f1e62f/IMG_5530.JPG Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/29cf708d-0fad-4395-9c66-785965564d38/IMG_7153.jpeg Lab News and Lab Fun! - Make it stand out Brooke presents her work at IZFS https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/efc5dd35-c755-418e-b8a6-8eb92dfcda28/IMG_4882.JPG Lab News and Lab Fun! - Make it stand out Candy’s work on mitochondria-ER contacts and neuronal mitochondrial maintenance was a big hit! https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/b7f7a13c-7362-4092-90bf-2cedddc2fbd9/IMG_4857.JPG Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593268878861-KB2QL5TUF1VG1NV1OS5D/IMG_1013.JPG Lab News and Lab Fun! First official lab picture! Summer of 2018. Left to right: Katie, Serena, Dane, Wesley, Kate, and Amrita. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1c51f425-2ebc-4b14-8bd2-de4a05907775/IMG_4014.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1593268959622-CAFO50LSKA5CC56DHVJN/image-asset.jpeg Lab News and Lab Fun! https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/ecd497d3-541b-4c84-b39a-964814b5512e/IMG_7161.jpeg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/ceac1074-ead4-4073-92f8-339024a9c441/escape+this.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1592784030945-9UWBBLGZCEKLNWCO9ZZ3/IMG_1807.jpg Lab News and Lab Fun! The lab in the fall of 2019 at the NICHD. Left to right - Front: Katie Drerup, Kate Pinter. Back: Serena Wisner, Amrita Mandal, Katie Klier. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/2061b993-1d74-4aa3-b805-c26ef52ae54c/tipsy+cow.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/5bb9d4d3-c943-44d9-88dc-e97db9a139c2/IMG_5452+2.JPG Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/826c9fdd-2262-415f-b7ec-a47ed8f85ba3/IMG_4886.JPG Lab News and Lab Fun! - Make it stand out Angelica telling the crowd about mitochondrial biogenesis https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/1610633107917-6XUBJOXBNNNP36NGDPJO/wong_WI_pic.jpg Lab News and Lab Fun! https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/560619e8-781d-415e-9c56-63412c1735d5/website+photo.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/c8cf9277-a256-47c1-ba43-57ee9673c4de/IMG_7158.jpeg Lab News and Lab Fun! - Make it stand out Brittany presents her work at IZFS https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/c24d8889-3a17-4c24-a4dc-e698fbf59715/IMG_1849.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5eee79d5489251622344aef4/a19fa9e2-2aaa-4a2d-bf89-b008c1f13f58/lake+fun.jpg Lab News and Lab Fun! - Make it stand out Whatever it is, the way you tell your story online can make all the difference.